BioVision 酵母菌 粒線體 Mitochondria 蛋白純化套組

 
BioVision提供酵母菌的粒線體蛋白純化套組, 只需要離心機, 就讓你輕鬆取得酵母粒線體蛋白!!

K259-50  http://www.biovision.com/yeast-mitochondria-isolation-kit-6284.html


Kit Summary:
Application:
• Isolation of highly pure intact mitochondria from yeast cells.
• Mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.
• Western blot and ELISA

Sample Type:
• Yeast cells culture.

Features & Benefits:
• Simple & convenient protocol
• The kit provides unique formulations of ready-to-use buffers and reagents to isolate intact mitochondria

Kit Components:
• Buffer A
• Buffer B
• 1 M DTT
• Homogenization Buffer
• Lysis Enzyme Mix
• Storage Buffer
• Protease Inhibitor Cocktail

還有相關產品

1111-50  Yeast Mitochondria (Pichia pastoris)
1111-200Yeast Mitochondria (Pichia pastoris)
1222-50  Yeast Mitochondria (S. cerevisiae)
1222-200Yeast Mitochondria (S. cerevisiae)


萊恩生物科技公司
台中區 04-25239639
業務專員  陳俊豪 0937-229803
電子信箱  biolion0819@gmail.com

Biovision 提供多種Protein Kinase Inhibitor 抑制劑研究

美國Biovision提供多種蛋白Kinase抑制劑

• Akt Inhibitors
• AMPK Inhibitors
• ATM Kinase Inhibitors
• Aurora Kinase Inhibitors
• CaM Kinase Inhibitors
• Casein Kinase (CK) Inhibitors
• CDK Inhibitors
• Chk Kinase Inhibitors
• DNA-PK Inhibitors
• GSK Inhibitors
• JAK Inhibitors
• JNK Inhibitors
• MAP Kinase/ MEK Inhibitors
• mTOR Inhibitors
• PI 3-Kinase Inhibitors
• PIM Kinase Inhibitors
• PIM1 Kinase Inhibitors
• Polo Like Kinase (PLK) Inhibitors
• Protein Kinase A (PKA) Inhibitors
• Protein Kinase C Inhibitors
• Protein Kinase G (PKG) Inhibitors
• Protein Tyrosine Kinase Inhibitors
• Raf Kinase Inhibitors
• Rho Kinase (ROCK) Inhibitors

 

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

Rapamycin、抑制免疫力之藥劑藥物用於各種各樣的疾病表示和在研究下在老化研究

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Biovision 提供多種老化試劑的研究, Rapamycin具有抑制免疫力之藥劑藥物用於各種各樣的疾病表示和在研究下在老化研究實驗室環球，被改進的功能和被擴大的生存在遭受導致膨脹的心肌病和少見肌肉 dystrophies (DCM) 在人的一個基因變化的鼠標。 當前沒有疾病的有效處理，包括剛玉粉Dreifuss 肌肉萎縮症和肢體帶肌肉萎縮症。Rapamycine只是許多研究抑制劑的一種

Biovision更提供多種研究抑制劑的需求!!

1568-5           Rapamycin                              5mg           US$195
1568-50         Rapamycin                             50mg          US$965
1746-5           EZSolution Rapamycin           5mg          US$195
3786-100       mTOR Antibody                    100ul          US$245

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

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BIOVISION提供Nuclear/Cytosol萃取試劑K266-100。有效萃取細胞核，細胞質蛋白。另有細胞膜，粒線體，plasma membrane等產品◦BIOVISION總代理商

多種細胞胞器蛋白質樣品萃取與製備

K267-50	Cytosol/Particulate Separation Kit	50 assays	$265.00
K270-50	FractionPREP™ Cell Fractionation Kit	50 assays	$395.00

K268-50	Membrane Protein Extraction Kit	50 assays	$335.00
K256-25	Mitochondria/Cytosol Fractionation Kit	25 assays	$135.00
K256-100	Mitochondria/Cytosol Fractionation Kit	100 assays	$325.00

K266-100	Nuclear/Cytosol Fractionation Kit	100 assays	$335.00
K266-25	Nuclear/Cytosol Fractionation Kit	25 assays	$135.00

 

線粒體/細胞質分離試劑盒

Mitochondria/Cytosol Fractionation Kit
Catalog#: K256-100

K256-25 Mitochondrial/Cytosol Fractionation Kit 25 assays

K256-100 Mitochondrial/Cytosol Fractionation Kit 100 assays

該試劑盒用於分離凋亡或非凋亡的哺乳動物細胞的細胞質和線粒體，可進行凋亡研究，或用Western Blot、ELISA來檢測，目標蛋白於細胞質和線粒體的localization情況。

Kit Summary:
• Detection method- Western blotting, ELISA, or other assays.
• Sample type- Cells and Tissues
• Species reactivity- Mammalian
• Kit size- 25, 100 assays
• Applications- Effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells.
Features & Benefits:
• Simple procedure; takes only 3-4 hours
• Fast and convenient
• The fractionation procedure is simple and staightforward. No ultracentrifugations are required. No toxic chemicals are involved.
Kit components:
• Mitochondria Extraction Buffer
• 5X Cytosol Extraction Buffer
• DTT ( 1 M )
• Protease Inhibitor Cocktail
Description:
The Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.

細胞核/細胞質分離試劑盒

Nuclear/Cytosol Fractionation Kit
Catalog#: K266-100

K266-25 Nuclear/Cytosol Fractionation Kit 25 assays

K266-100 Nuclear/Cytosol Fractionation Kit 100 assays

試劑盒內包含，分離哺乳動物細胞的細胞核和細胞質所有試劑，不僅能夠分離細胞核和細胞質蛋白質，而且可以保持蛋白質的完整性。所分離的細胞核和細胞質蛋白質可進行轉錄活性、RNA 剪接、凝膠阻滯電泳實驗、報告蛋白檢測、酶活性檢測及Western Blot 研究等。

Kit Summary:
• Sample type- Cell culture (adherent and suspension), fresh and frozen tissues
• Species reactivity- Mammalian
• Kit size- Conveient sizes (25, 100)
• Applications- This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells and tissues The new kit provides a system that maintains the nuclear and cytoplasmic compartments separate and intact. Transcriptional activity/reporter assays/enzyme activity assays/Western blotting and more downstream assays.
Features & Benefits:
• Simple procedure; takes less than 2-3 hours
• Fast and convenient
• The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic extracts from cells relatively quick with little or no cross-contaminations
Kit components:
• Cytosol Extraction Buffer A (CEB-A)
• Cytosol Extraction Buffer B (CEB-B)
• Nuclear Extraction Buffer (NEB)
• DTT ( 1 M )
• Protease Inhibitor Cocktail (lyophilized)
Description:
This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.

細胞質/胞內顆粒快速分離試劑盒

K267-50 Cytosol/Particulate Rapid Separation Kit 50 assays

試劑盒利用物理學原理，通過一個油層將細胞質和胞內顆粒快速分開，使得兩個蛋白質不能互相接觸或彌散，進而實現對蛋白質進行精確的亞細胞定位和分析。

膜蛋白抽提試劑盒

K268-50 Membrane Protein Extraction Kit 50 assays

該試劑盒提供最佳化的緩衝液和試劑，用來高效地萃取哺乳動物組織和細胞的膜蛋白。傳統的方法或試劑盒僅能提取總的細胞膜蛋白，包括漿膜和細胞器膜蛋白。該試劑盒不僅能分離總的細胞膜蛋白，還可以單獨純化漿膜蛋白(plasma membrane protein)。試劑盒所抽提出的膜蛋白可用於Western Blot，二維電泳和酶活性分析等多種檢測方法。

哺乳細胞蛋白抽提試劑盒

Membrane Protein Extraction KitCatalog#: K268-50

K269-500 Mammalian Cell Extraction Kit 500 assays

該試劑盒內含有最佳化的緩衝液和試劑，用於從培養細胞和組織中非變性地萃取哺乳動物蛋白，所得到的細胞裂解物可用來酶活性的檢測 (如Caspase 活性測試)、Western Blot 等多種用途。

Kit Summary:
• Sample type- Cell culture (adherent and suspension), fresh and frozen tissues
• Species reactivity- Mammalian
• Applications- Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.
Features & Benefits:
• Simple procedure
• Fast and convenient
• The Membrance Protein Extraction Kit provides an easy and complete protocol for isolating integral membrance proteins efficiently from cultured mammalian cells.
Kit components:
• Homogenize Buffer
• Upper Phase Solution
• Lower Phase Solution
• Protease Inhibitor Cocktail
Description:
The Membrane Protein Extraction Kit provides optimized buffers and reagents for effective extraction of membrane proteins from mammalian tissues and cells. Unlike other available procedures that can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins), BioVision’s kit was designed to not only extract the total cellular membrane proteins, but also purify the plasma membrane proteins specifically. The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

四合一胞器分離試劑盒

FractionPREP™ Cell Fractionation Kit

K270-50 Fraction-PREPTM Cell Fractionation System 50 assays

該試劑盒能從一個哺乳動物樣本中抽提出四種蛋白質，細胞質、細胞核、膜/顆粒及細胞骨架組分等。方法簡單、快速，整個過程只需2 小時；無需超速離心；結果重複性好； 所分離的蛋白質可用於一維或二維電泳。

Kit Summary:
• Sample type- Cell culture (adherent and suspension), fresh and frozen tissues
• Species reactivity- Mammalian
• Applications- 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Features & Benefits:
• Simple procedure; takes less than 1 hour
• Fast and convenient
• The kit is designed for serial sample preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions, from one sample.
Kit components:
• Cytosol Extraction Buffer (CEB)
• Membrane Extraction Buffer-A (MEB-A)
• Membrane Extraction Buffer-B (MEB-B)
• Nuclear Extraction Buffer (NEB)
• DTT ( 1 M )
• Protease Inhibitor Cocktail (lyophilized)
Description:
This FractionPREP™ Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

EZLys lysozyme菌液萃取蛋白質#8005-1G。可水解細菌細胞壁peptidoglycan 的糖鍵，使菌體無法承受滲透壓逆境而破裂，改良蛋白質過程造成的傷害。【萊恩生物科技有限公司】

EZLys(TM) Lysozyme, Human

Catalog#: 8005 -1G | Size: 1 g

產品介紹

lysozyme 可水解細菌細胞壁中 peptidoglycan 的糖鍵，使得菌體無法承受滲透壓逆境而破裂，可改良蛋白質過程造成的damage；但加入lysozyme前要先加入cell extraction buffer。最佳化狀態須加入protease inhibitors,及lysozyme 可以降低蛋白質降解粘稠度及改良萃取過程中的傷害。 EZLys(TM)菌液萃取蛋白質試劑, 500ml，其可以萃取native及non-denatured form蛋白質。產品有提供soluble片段及inclusion body片段的操作方法。如附件所示。

A proprietary formulation prepared in a 50 mM Tris pH8.0 buffer and designed for gentle

disruption of cell membrane to extract proteins. It provides a simple, rapid, low cost method for releasing expressed target protein for preparation, purification, or other applications. The EZLys™ reagent is an optically transparent, non-denaturing formulation which can be easily removed by dialysis. It is also useful for the preparation of high purity inclusion bodies for subsequent purification. This reagent does not interfere with typical protein immobilization or cross linking and is suitable for use in the BCA or Bradford protein assays. Ideal pH range is pH 5 -10. The 100 ml and 500 ml reagent are sufficient for protein extraction from 25 g and 125 g of cell paste.

產品特點

1) Complete cell lysis without mechanical stress which is tedious and can damage proteins.

2) The extracted proteins are in native, non-denatured form.

3) The reagent does not interfere with down-stream functional testing or protein assays.

4) The reagent can easily be removed by dialysis or spin-column buffer exchange.

Alternate Names / Synonyms: Protein Extraction

Gene Symbol: N/A

Accession #: N/A

Gene ID: N/A

Source: E. coli

Appearance: Off- white powder

Physical Form Description: Off- white powder

Molecular Weight: N/A

Purity by SDS-PAGE: ≥85%

Purity by HPLC: N/A

Endotoxin Level: N/A

Biological Activity: N/A

Reconstitution Instructions: Clear solution at 10 mg/ml in PBS, pH 7.4 or water

Storage Temp.: -20°C

Shipping: Gel Pack

更多參考內容:

原廠連結: http://www.biovision.com/

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

改良版的Coomassie Brilliant Blue G,蛋白質定量套組【萊恩生物科技有限公司】04-25239639

此定量方法利用改良版的Coomassie Brilliant Blue G會與蛋白質結合的特性，在G與蛋白質結合後，G的顏色會從紅色轉變成為藍色，此時在595 nm波長下，會有較高的吸收，以便偵測。這個方法的優點為G與蛋白質結合所需的時間很短(大約2分鐘左右)，且結合的G蛋白質複合物(complex)可在溶液中維持較長的時間(大約1小時)。此法方便而靈敏(10 ng 左右)，且可使用微量滴定盤進行分析，可減低試劑用量，方便大量樣本的操作。

Catalog#: K810-1000 | Size: 1000 assays

Kit Summary:
• Detection method- Absorbance (595 nm)
• Sample type- Cell and Tissue culture homogenates (eukaryotic cells), serum, plasma and other samples contaning proteins
• Species reactivity- Mammalian
• Application- The Protein Quantification Kit provides a simple and rapid procedure for determining the concentration of protein in solution.

Features & Benefits:
• Simple procedure; takes ~ 20 minutes
• Fast and convenient

Kit components:
• Protein Assay Solution (5X)
• Protein Standard (1 mg/ml)

Description:
The Protein Quantification Kit provides a simple and rapid procedure for determining the concentration of protein in solution. The method utilizes an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.

Storage Conditions:
+ 4°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

Kit Summary:
• Detection method- Absorbance (562 nm)
• Sample type- Cell and Tissue culture homogenates (eukaryotic cells), serum, plasma
• Species reactivity- Mammalian
• Application- Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).

Features & Benefits:
• Simple procedure; takes ~ 30-90 minutes
• Fast and convenient

Kit components:
• BCA Reagent
• Copper Reagent
• BSA Standard (10 mg/ml)

Description:
The BCA Protein Assay Kit provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. The method utilizes a copper (Cu²ᶧ) salt which can be reduced to the cuprous state by protein(s). The generated Cu²ᶧ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm. The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).

Storage Conditions:
+ 4°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

更多參考內容:

原廠連結: http://www.BIOVISION.com/

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

【Ni-IDA His tag標誌蛋白質純化 agarose】abmgood# G250, Ni-IDA Agarose Beads 重組蛋白純化

Ni-IDA Agarose Resins for Protein Purification	5ml	G250
Ni-IDA Agarose Resins for Protein Purification	25ml	G251
Ni-IDA Agarose Resins for Protein Purification	100ml	G253
Ni-IDA Agarose 1ml Column for Protein Purification	8x1ml	G239
Ni-IDA Agarose 5ml Column for Protein Purification	5x5ml	G240

Description

Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni²⁺ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.

Applications

 Purification of His-tagged proteins.

 Antibody purification.

 Assays involving protein-protein and protein-DNA interactions.

 Structural and functional investigation of proteins.

Special Features

 One-step easy purification protocol from crude lysate to >95% pure protein.

 Purification under native or denaturing conditions.

 Ready-to-use for any scale of purification.

 Protein binding capacity: approx. 20-40 μmol Ni²⁺/ml gel.

 Economical value.

 Can be used for successive cycles.

 Resins and columns formats suitable for batch and gravity purification.

Related Products

Purified protein fractions can be detected using Anti-HIS-tag Antibody

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com