【Ni-IDA His tag標誌蛋白質純化 agarose】abmgood# G250, Ni-IDA Agarose Beads 重組蛋白純化

Ni-IDA Agarose Resins for Protein Purification	5ml	G250
Ni-IDA Agarose Resins for Protein Purification	25ml	G251
Ni-IDA Agarose Resins for Protein Purification	100ml	G253
Ni-IDA Agarose 1ml Column for Protein Purification	8x1ml	G239
Ni-IDA Agarose 5ml Column for Protein Purification	5x5ml	G240

Description

Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni²⁺ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.

Applications

 Purification of His-tagged proteins.

 Antibody purification.

 Assays involving protein-protein and protein-DNA interactions.

 Structural and functional investigation of proteins.

Special Features

 One-step easy purification protocol from crude lysate to >95% pure protein.

 Purification under native or denaturing conditions.

 Ready-to-use for any scale of purification.

 Protein binding capacity: approx. 20-40 μmol Ni²⁺/ml gel.

 Economical value.

 Can be used for successive cycles.

 Resins and columns formats suitable for batch and gravity purification.

Related Products

Purified protein fractions can be detected using Anti-HIS-tag Antibody

【供應廠商】萊恩生物科技有限公司

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