EZLys(TM) Lysozyme, Human
產品介紹
lysozyme 可水解細菌細胞壁中 peptidoglycan 的糖鍵，使得菌體無法承受滲透壓逆境而破裂，可改良蛋白質過程造成的damage；但加入lysozyme前要先加入cell extraction buffer。最佳化狀態須加入protease inhibitors,及lysozyme 可以降低蛋白質降解粘稠度及改良萃取過程中的傷害。 EZLys(TM)菌液萃取蛋白質試劑, 500ml，其可以萃取native及non-denatured form蛋白質。產品有提供soluble片段及inclusion body片段的操作方法。如附件所示。
A proprietary formulation prepared in a 50 mM Tris pH8.0 buffer and designed for gentle
disruption of cell membrane to extract proteins. It provides a simple, rapid, low cost method for releasing expressed target protein for preparation, purification, or other applications. The EZLys™ reagent is an optically transparent, non-denaturing formulation which can be easily removed by dialysis. It is also useful for the preparation of high purity inclusion bodies for subsequent purification. This reagent does not interfere with typical protein immobilization or cross linking and is suitable for use in the BCA or Bradford protein assays. Ideal pH range is pH 5 -10. The 100 ml and 500 ml reagent are sufficient for protein extraction from 25 g and 125 g of cell paste.
產品特點
1) Complete cell lysis without mechanical stress which is tedious and can damage proteins.
2) The extracted proteins are in native, non-denatured form.
3) The reagent does not interfere with down-stream functional testing or protein assays.
4) The reagent can easily be removed by dialysis or spin-column buffer exchange.
Alternate Names / Synonyms: Protein Extraction
Gene Symbol: N/A
Accession #: N/A
Gene ID: N/A
Source: E. coli
Appearance: Off- white powder
Physical Form Description: Off- white powder
Molecular Weight: N/A
Purity by SDS-PAGE: ≥85%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: Clear solution at 10 mg/ml in PBS, pH 7.4 or water
Storage Temp.: -20°C
Shipping: Gel Pack
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