多種細胞胞器蛋白質樣品萃取與製備
| K267-50 | Cytosol/Particulate Separation Kit | 50 assays | $265.00 | |
| K270-50 | FractionPREP™ Cell Fractionation Kit | 50 assays | $395.00 |
| K268-50 | Membrane Protein Extraction Kit | 50 assays | $335.00 | |
| K256-25 | Mitochondria/Cytosol Fractionation Kit | 25 assays | $135.00 | |
| K256-100 | Mitochondria/Cytosol Fractionation Kit | 100 assays | $325.00 |
| K266-100 | Nuclear/Cytosol Fractionation Kit | 100 assays | $335.00 | |
| K266-25 | Nuclear/Cytosol Fractionation Kit | 25 assays | $135.00 |
Mitochondria/Cytosol Fractionation Kit
Catalog#: K256-100
Catalog#: K256-100
K256-25 Mitochondrial/Cytosol Fractionation Kit 25 assays
K256-100 Mitochondrial/Cytosol Fractionation Kit 100 assays
該試劑盒用於分離凋亡或非凋亡的哺乳動物細胞的細胞質和線粒體，可進行凋亡研究，或用Western Blot、ELISA來檢測，目標蛋白於細胞質和線粒體的localization情況。
Kit Summary: • Detection method- Western blotting, ELISA, or other assays.
• Sample type- Cells and Tissues
• Species reactivity- Mammalian
• Kit size- 25, 100 assays
• Applications- Effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells.
Features & Benefits:
• Simple procedure; takes only 3-4 hours
• Fast and convenient
• The fractionation procedure is simple and staightforward. No ultracentrifugations are required. No toxic chemicals are involved.
Kit components:
• Mitochondria Extraction Buffer
• 5X Cytosol Extraction Buffer
• DTT ( 1 M )
• Protease Inhibitor Cocktail
Description:
The Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
細胞核/細胞質分離試劑盒
Nuclear/Cytosol Fractionation Kit
Catalog#: K266-100
Catalog#: K266-100
K266-25 Nuclear/Cytosol Fractionation Kit 25 assays
K266-100 Nuclear/Cytosol Fractionation Kit 100 assays
試劑盒內包含，分離哺乳動物細胞的細胞核和細胞質所有試劑，不僅能夠分離細胞核和細胞質蛋白質，而且可以保持蛋白質的完整性。所分離的細胞核和細胞質蛋白質可進行轉錄活性、RNA 剪接、凝膠阻滯電泳實驗、報告蛋白檢測、酶活性檢測及Western Blot 研究等。
Kit Summary: • Sample type- Cell culture (adherent and suspension), fresh and frozen tissues
• Species reactivity- Mammalian
• Kit size- Conveient sizes (25, 100)
• Applications- This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells and tissues The new kit provides a system that maintains the nuclear and cytoplasmic compartments separate and intact. Transcriptional activity/reporter assays/enzyme activity assays/Western blotting and more downstream assays.
Features & Benefits:
• Simple procedure; takes less than 2-3 hours
• Fast and convenient
• The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic extracts from cells relatively quick with little or no cross-contaminations
Kit components:
• Cytosol Extraction Buffer A (CEB-A)
• Cytosol Extraction Buffer B (CEB-B)
• Nuclear Extraction Buffer (NEB)
• DTT ( 1 M )
• Protease Inhibitor Cocktail (lyophilized)
Description:
This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
細胞質/胞內顆粒快速分離試劑盒
K267-50 Cytosol/Particulate Rapid Separation Kit 50 assays
試劑盒利用物理學原理，通過一個油層將細胞質和胞內顆粒快速分開，使得兩個蛋白質不能互相接觸或彌散，進而實現對蛋白質進行精確的亞細胞定位和分析。
膜蛋白抽提試劑盒
K268-50 Membrane Protein Extraction Kit 50 assays
該試劑盒提供最佳化的緩衝液和試劑，用來高效地萃取哺乳動物組織和細胞的膜蛋白。傳統的方法或試劑盒僅能提取總的細胞膜蛋白，包括漿膜和細胞器膜蛋白。該試劑盒不僅能分離總的細胞膜蛋白，還可以單獨純化漿膜蛋白(plasma membrane protein)。試劑盒所抽提出的膜蛋白可用於Western Blot，二維電泳和酶活性分析等多種檢測方法。
哺乳細胞蛋白抽提試劑盒
Membrane Protein Extraction KitCatalog#: K268-50
K269-500 Mammalian Cell Extraction Kit 500 assays
該試劑盒內含有最佳化的緩衝液和試劑，用於從培養細胞和組織中非變性地萃取哺乳動物蛋白，所得到的細胞裂解物可用來酶活性的檢測 (如Caspase 活性測試)、Western Blot 等多種用途。
Kit Summary: • Sample type- Cell culture (adherent and suspension), fresh and frozen tissues
• Species reactivity- Mammalian
• Applications- Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.
Features & Benefits:
• Simple procedure
• Fast and convenient
• The Membrance Protein Extraction Kit provides an easy and complete protocol for isolating integral membrance proteins efficiently from cultured mammalian cells.
Kit components:
• Homogenize Buffer
• Upper Phase Solution
• Lower Phase Solution
• Protease Inhibitor Cocktail
Description:
The Membrane Protein Extraction Kit provides optimized buffers and reagents for effective extraction of membrane proteins from mammalian tissues and cells. Unlike other available procedures that can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins), BioVision’s kit was designed to not only extract the total cellular membrane proteins, but also purify the plasma membrane proteins specifically. The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.
四合一胞器分離試劑盒
FractionPREP™ Cell Fractionation Kit
K270-50 Fraction-PREPTM Cell Fractionation System 50 assays
該試劑盒能從一個哺乳動物樣本中抽提出四種蛋白質，細胞質、細胞核、膜/顆粒及細胞骨架組分等。方法簡單、快速，整個過程只需2 小時；無需超速離心；結果重複性好； 所分離的蛋白質可用於一維或二維電泳。
Kit Summary: • Sample type- Cell culture (adherent and suspension), fresh and frozen tissues
• Species reactivity- Mammalian
• Applications- 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Features & Benefits:
• Simple procedure; takes less than 1 hour
• Fast and convenient
• The kit is designed for serial sample preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions, from one sample.
Kit components:
• Cytosol Extraction Buffer (CEB)
• Membrane Extraction Buffer-A (MEB-A)
• Membrane Extraction Buffer-B (MEB-B)
• Nuclear Extraction Buffer (NEB)
• DTT ( 1 M )
• Protease Inhibitor Cocktail (lyophilized)
Description:
This FractionPREP™ Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting
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