此定量方法利用改良版的Coomassie Brilliant Blue G會與蛋白質結合的特性，在G與蛋白質結合後，G的顏色會從紅色轉變成為藍色，此時在595 nm波長下，會有較高的吸收，以便偵測。這個方法的優點為G與蛋白質結合所需的時間很短(大約2分鐘左右)，且結合的G蛋白質複合物(complex)可在溶液中維持較長的時間(大約1小時)。此法方便而靈敏(10 ng 左右)，且可使用微量滴定盤進行分析，可減低試劑用量，方便大量樣本的操作。
Catalog#: K810-1000 | Size: 1000 assays
Kit Summary:
• Detection method- Absorbance (595 nm)
• Sample type- Cell and Tissue culture homogenates (eukaryotic cells), serum, plasma and other samples contaning proteins
• Species reactivity- Mammalian
• Application- The Protein Quantification Kit provides a simple and rapid procedure for determining the concentration of protein in solution.
• Detection method- Absorbance (595 nm)
• Sample type- Cell and Tissue culture homogenates (eukaryotic cells), serum, plasma and other samples contaning proteins
• Species reactivity- Mammalian
• Application- The Protein Quantification Kit provides a simple and rapid procedure for determining the concentration of protein in solution.
Features & Benefits:
• Simple procedure; takes ~ 20 minutes
• Fast and convenient
• Simple procedure; takes ~ 20 minutes
• Fast and convenient
Kit components:
• Protein Assay Solution (5X)
• Protein Standard (1 mg/ml)
• Protein Assay Solution (5X)
• Protein Standard (1 mg/ml)
Description:
The Protein Quantification Kit provides a simple and rapid procedure for determining the concentration of protein in solution. The method utilizes an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.
The Protein Quantification Kit provides a simple and rapid procedure for determining the concentration of protein in solution. The method utilizes an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.
Storage Conditions:
+ 4°C
+ 4°C
Shipping Conditions:
gel pack
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
Kit Summary:
• Detection method- Absorbance (562 nm)
• Sample type- Cell and Tissue culture homogenates (eukaryotic cells), serum, plasma
• Species reactivity- Mammalian
• Application- Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).
• Detection method- Absorbance (562 nm)
• Sample type- Cell and Tissue culture homogenates (eukaryotic cells), serum, plasma
• Species reactivity- Mammalian
• Application- Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).
Features & Benefits:
• Simple procedure; takes ~ 30-90 minutes
• Fast and convenient
• Simple procedure; takes ~ 30-90 minutes
• Fast and convenient
Kit components:
• BCA Reagent
• Copper Reagent
• BSA Standard (10 mg/ml)
• BCA Reagent
• Copper Reagent
• BSA Standard (10 mg/ml)
Description:
The BCA Protein Assay Kit provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. The method utilizes a copper (Cu²ᶧ) salt which can be reduced to the cuprous state by protein(s). The generated Cu²ᶧ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm. The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).
The BCA Protein Assay Kit provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. The method utilizes a copper (Cu²ᶧ) salt which can be reduced to the cuprous state by protein(s). The generated Cu²ᶧ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm. The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).
Storage Conditions:
+ 4°C
+ 4°C
Shipping Conditions:
gel pack
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
更多參考內容:
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