Invent 快速column filter蛋白純化套組

Minute™蛋白萃取套組，使用創新Column Filter技術，效果更佳。

 

優點：

蛋白萃取只需要vortex、transfer、spin。

簡單四步驟完成，像相較同類型產品節省6-12個步驟，降低人為操作失誤。

無須長時間等待，節省90%時間。

免除重複wash步驟。免超音波震盪、避免蛋白加熱與變性。

 

針對磷酸化實驗，在蛋白質萃取過程中可以維持蛋白質原有的磷酸化狀況。

小分子蛋白亦可獲得較多保留。

 

應用：SDS-PAGE、Immunoblottings、ELISA、IP、Protein localization、Gel mobility shift assay等。並有Denature/native，Detergent free套組可選擇，可用於MASS、HPLC等實驗。

 



 

萊恩生物科技公司

[連絡電話] : 04-25239639

[台中專員]   陳俊豪

[行動電話] : 0937229803

[電子信箱] : biolion0819@gmail.com

 

His-tag 純化相關耗材, 新產品His spin column快速純化His-tag蛋白, 最高可純化2.8mg protein / per spin column

Biovision 提供快速純化His-tag試劑套組, ready to use更方便小量蛋白純化分析!!

新產品Ready-to-Use Ni-IDA Spin Purification Kit 快速純化His-tag蛋白, 最高可純化2.8mg protein  per spin column

Katalog No: K6567-25

Product Name: Ready-to-Use Ni-IDA Spin Purification Kit

Product Highlights:
Salient Features:
Binding Capacity: Upto 2.8 mg poly-histidine tagged proteins per column.
Column Content: 25 Ni-IDA spin columns, each containing 100 µl high performance Hi-Bind™ Ni QR Agarose Beads (Cat. #6562).
Bead content: A 50% slurry (e.g., 1 ml of settled resin is equivalent to 2 ml of 50% slurry) in 20% ethanol.
Applications:
• Ideal for small scale, one – step purification of poly-histidine tagged recombinant proteins under native conditions.
• Ideal for small scale, one – step purification of native proteins and peptides that have an affinity for metal ions.
• Screening of expression levels in a HTP manner.
• Applicable for purification under native and denaturing conditions.

Description:
Protein activity and functional studies rely largely on the availability of purified target proteins. Majority of protein studies are carried out on tagged recombinant proteins expressed in various host organisms. Over 50% of these tagged recombinant proteins are expressed as fusions with poly-histidine purification tags. The small size and the mild conditions utilized during His-tagged protein purification as well as the associated low costs makes this type of fusion the most popular (and in many cases, the first tag of choice). BioVision’s Ready-to-Use Ni-IDA Spin Purification Kit provides an all-inclusive set of reagents required for the small-scale purification of these poly-histidine-tagged proteins rapidly. Each of the Ni-IDA Spin Columns is filled with 100 µL of high performance Hi-Bind™ Ni QR Agarose Beads (Cat. #6562), enabling efficient capture and purification of up to 2.8 mg of poly-histidine tagged proteins per column. The small volume of beads inside the columns is optimal for loading various amounts of sample. At normal expression levels, it permits efficient displacement of non-tagged protein impurities by the more strongly binding poly-histidine tagged targets and improves the purity of the final product. The beads packed in each column deliver as much as 25% higher capacity than Ni-NTA adsorbents while dramatically decreasing purification times due to the ability to perform centrifugation. The kit also includes ready to use column loading, wash and elution buffers aiding in fast purification of the His-tagged proteins. The yield and purity of purification are protein specific and depends on various factors like the expression level, structure, solubility etc.

Storage Temp.:+4°C

 相關耗材resin提供參考!!

6562-1	Hi-Bind™ Ni QR Agarose Beads	1 ml
6562-10	Hi-Bind™ Ni QR Agarose Beads	10 ml
6562-100	Hi-Bind™ Ni QR Agarose Beads	100 ml
6562-500	Hi-Bind™ Ni QR Agarose Beads	500 ml
6567-25	Ni-IDA Spin Columns	25 columns
K6563-3	Ready-to-Use Ni QR Agarose Beads Buffer Kit	3 components

萊恩生物科技公司

訂購電話:  04-25239639

台中區專員   陳俊豪

行動電話:  0937-229803

Biovision提供蛋白酶(酵素)抑制劑 Enzyme inhibitor 經銷商 " 萊恩生物科技有限公司"

BioVision公司提供的800多種蛋白沒酶抑製劑，涵蓋所有的核心生物/藥理學研究領域的全面收集。 不同類型的抑製劑被用來研究生物與醫藥疾病，導致發現新的藥物或改善現有藥物的最終提高人類的疾病，如愛滋病，癌症，糖尿病，老年癡呆症的治療方案列表是不斷增加的。

ACAT Inhibitors

ACC2 Inhibitors

Adenylase Cyclase Inhibitors

Aldo-Keto Reductase (AKR) Inhibitors

Angiogenesis Inhibitors

Angiotensin Receptor Antagonists

Arginase Inhibitors

ATPase Inhibitors

Autophagy Inhibitors

Bcl-2 Inhibitors

BMP Inhibitors

Bone Resorption Inhibitors

Bromodomain Inhibitors

Calpain Inhibitors

Caspase & Granzyme B Inhibitors

Cathepsin Inhibitors

CDc42 Inhibitors

CETP Inhibitors

Cyclooxygenase (COX) Inhibitors

Dipeptidyl peptidase-4 (DPP-4) Inhibitors

DUBs (Deubiquitinating Enzymes) and related Inhibitors

FAAH Inhibitors

Farnesyl Protein Transferase (FPT) Inhibitors

Fatty Acid Synthase (FAS) Inhibitors

Ferroptosis Inhibitors

Glucosidase Inhibitors

GST Inhibitors

Heat Shock Protein (Hsp) Inhibitors

Hedgehog (Hh) Signaling Pathway Antagonists

Histone Acetyltransferase (HAT) Inhibitors

Histone Deacetylase (HDAC) Inhibitors

Histone Demethylase (HDM) Inhibitors

HIV Integrase Inhibitors

HMG-CoA Inhibitors

Hypoxia Inducible Factor (HIF) Inhibitors

Isocitrate Dehydrogenase (IDH) Inhibitors

KMO (Kynurenine 3-monoxygenase) Inhibitors

Matrix Metalloproteinase (MMP) Inhibitors

MDM2 Inhibitors

Methyltransferase Inhibitors

Microtubule Inhibitors

MIF Antagonists

Mitochondrial Division Inhibitors

Mitochondrial Electron Transport Chain Inhibitors

Monoamine oxidase (MAO) Inhibitors

Myeloperoxidase (MPO) Inhibitors

Necroptosis and Necrosis Inhibitors

NF-KB Pathway Inhibitors

Nitric Oxide Synthase (NOS) Inhibitors

Nuclear Export Inhibitors

p53 Inhibitors

Phosphatase Inhibitors

Phosphodiesterase (PDE) Inhibitors

Poly (ADP-ribose) Polymerase (PARP) Inhibitors

PPAR Antagonists

Protease Inhibitors

Proteasome Inhibitors

Protein Kinase Inhibitors

PTEN Inhibitors

Rac1 Inhibitors

Renin Inhibitors

SCD1 Inhibitors

Secretase Inhibitors

SIRT Inhibitors

Smad Inhibitors

Sphingosine Kinase (SK) Inhibitors

Stat Inhibitors

TACE Inhibitors

Thioredoxin inhibitors

Topoisomerase Inhibitors

Ubiquitin Activating Enzyme E1 Inhibitor

Ubiquitin Ligase (E3) Inhibitors

Ubiquitin Proteasome Pathway Inhibitor

Ubiquitin Proteasome Pathway Inhibitors

Wnt Signaling Inhibitors

Other Inhibitors     供應廠商: 萊恩生物科技有限公司   訂貨專線: 04-25239639   台中專員: 陳俊豪   行動電話: 0937-229803   電子信箱: biolion0819@gmail.com

Biovision 超高靈敏度蛋白質定量套組 Extra Sense™ BCA Protein Assay Kit

Extra Sense™ BCA Protein Assay Kit
The most sensitive kit on the market

Catalog#: K814-2500  | Size: 2500 assays

Catalog#: K814-5000  | Size: 5000 assays

Biovision提供高靈敏度蛋白質定量試劑組, 線性度可再0.5-40 μg/ml的範圍內針對低濃度蛋白樣品, 或是珍貴的樣品不想做太多的消耗蛋白定量前步驟實驗, Biovision 可以輕鬆解決您的問題!!

 


Assay Kit Name:
Extra Sense™ BCA Protein Assay Kit

Kit Summary:
• Detection method- Absorbance (562 nm)
• Detergent compatible

Sample Type:
N/A

Features & Benefits:
• Simple & convenient
• optimized for determining total protein content in low protein concentration samples (0.5-40 μg/ml)

Kit Components:
• BCA Reagent A
• BCA Reagent B
• BCA Reagent C
• BSA (2 mg/ml)

Description:
BioVision’s Extra Sense™ BCA Protein Assay kit provides an ultra-sensitive method to detect and quantify total protein concentration even in the presence of detergents. The Kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/ml). The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. The Cu+1-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. The Kit includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.

Storage Conditions:
RT
 
另外還有一般型BCA定量套組

BCA Protein Assay Kit II

Catalog#: K813-2500  | Size: 2500 assays

Catalog#: K813-5000  | Size: 5000 assays

Assay Kit Name:
BCA Protein Assay Kit II

Kit Summary:
Detection method- Absorbance (562 nm)
Detergent compatible  

Sample Type:
N/A  

Features & Benefits:
• Simple & convenient
• High linearity: linear over a wide range of protein concentrations between 25-2000 μg/ml.  

Kit Components:
• BCA Reagent A
• BCA Reagent B
• BSA Standard (2 mg/ml)  

Description:
Biovision’s BCA Protein Assay kit is useful for colorimetric detection and quantification of total protein content even in the presence of detergents. The Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline medium. The amount of purple-colored product formed during the reaction can be correlated to the amount of protein in the assay. The Cu+1-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. This BCA Protein Assay Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of the samples.  

Storage Conditions:
RT 

相關連結

http://www.biovision.com/extra-sense-bca-protein-assay-kit-6786.html

http://www.biovision.com/bca-protein-assay-kit-ii-6784.html

供應廠商   萊恩生物科技有限公司

訂貨專線   04-2539639

台中專員   陳俊豪 0937-229803

電子信箱   biolion0819@gmail.com

蛋白質抑制劑Protease inhibitor and 磷酸酶蛋白抑制劑phosphatase相關產品

您需要蛋白質抑制劑 Protease inhibitor嗎??
    還是磷酸酶抑制劑 Phosphatase inhibitor呢??
 
給您方便使用的專家, 請找萊恩生物科技有限公司
     只需稀釋就讓您的蛋白乖乖聽話!!

K271-500

Protease Inhibitor Cocktail

K272-1EA

EZBlock Protease Inhibitor Cocktail, EDTA-Free

K277-1EA

EZBlock™ Protease Inhibitor Cocktail II

K278-1EA

EZBlock™ Protease Inhibitor Cocktail III

K279-1

EZBlock™ Protease Inhibitor Cocktail IV

K279-1EA

EZBlock™ Protease Inhibitor Cocktail IV

K272-1

EZBlock™ Protease Inhibitor Cocktail, EDTA-Free

K272-5

EZBlock™ Protease Inhibitor Cocktail, EDTA-Free

K283-1

EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail

K284-1

EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail , EDTA-Free

K284-1EA

EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail , EDTA-Free

提供廠商:  萊恩生物科技公司 聯絡專線:  04-25239639 台中區專員  陳俊豪  0937-229803

粒線體蛋白免疫沉澱分析套組 Mitochondrial Protein IP Kit 研究套組

線粒體功能障礙會導致一些疾病，如心功能不全，糖尿病，延緩衰老和神經系統疾病，主要是由線粒體DNA基因突變引起的。

BioVision提供粒線體蛋白免疫沉澱分析套組 Mitochondrial Protein IP Kit 研究套組

相關連結 http://www.biovision.com/mitochondrial-protein-ip-kit-6285.html

Kit Summary:
Application:
• Optimized for compatibility with immunoprecipitation (IP and co-IP) and pull-down using tagged proteins.
• Gentle formulation for maintenance of stable mitochondrial complexes.
• Compatible with SDS PAGE, 2D gel, Blue-Native gel, Mass spectrometry.
• Functional Assays and enzymatic assays.  Sample Type:
• Mitochondria, mitochondrial extract or cell lysate.  Features & Benefits:
• Simple & convenient protocol
• The Kit provides unique formulation of ready-to-use buffers & reagents for IP/Co-IP/Pull down of mitochondrial proteins or complexes.

Kit Components:
• Mitochondria Protein IP Buffer
• Wash Buffer (5X)
• Protease Inhibitor Cocktail
• 10% n-Dodecyl-beta-D-maltoside
• 10% Triton X-100
• 10% Digitonin  Description:
Mitochondria are the power house of the cells and play an essential role in energy production. Damage to the mitochondria activates signaling pathways that induce apoptosis. Mitochondria also regulate and mediate transport of the metabolites and ions needed for oxidative phosphorylation and maintenance of membrane potential for ATP synthesis. Mitochondrial dysfunction leads to several disorders like cardiac dysfunction, diabetes, aging and neurological disorder, mainly caused by mutations in mitochondrial DNA or in nuclear genes that code for mitochondrial components. Thus, mitochondria have several different functions in the cell.
BioVision ready to use mitochondria Protein IP Buffer is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.

提供公司     萊恩生物科技有限公司

台中電話     04-25239639

聯絡業務     陳俊豪 0937-229803

電子信箱     biolion0819@gmail.com

新產品 免疫沉澱套組Immunoprecipitation (IP) Kit 針對 IP/Co-IP of proteins/complexes 功能研究與分析

提供Immunoprecipitation (IP) Kit  針對 IP/Co-IP of proteins/complexes 功能研究與分析
BioVision的免疫沉澱試劑盒提供緩衝溶液準備細胞/組織提取物，抗原結合和洗滌步驟。蛋白A/ G瓊脂糖珠套件中提供比傳統的蛋白A或蛋白G樹脂結合具有更高的結合能力之試劑。該試劑盒可用於多種免疫沉澱或合作IP研究。

相關連結 http://www.biovision.com/immunoprecipitation-ip-kit-6422.html

Kit Summary:
Application:
• Immunoprecipitation (IP) and Co-IP
• Functional study of Immunoprecipitated proteins/complexes.
• SDS-PAGE or western blot analysis of Immunoprecipitated proteins/complexes Sample Type:
• Tissue or cell extracts
• Biological samples Features & Benefits:
• Provides optimized buffers for preparing cell/tissue extracts, antigen binding and washing steps
• The protein A/G Sepharose beads provided in the kit has higher binding capacity with broader antibody isotype binding than traditional protein A or protein G resins.
• Validated for functional assays as well as analysis of IP/Co-IP proteins/complexes Kit Components:
• Non-Denaturing Lysis Buffer
• RIPA Lysis Buffer
• Protease Inhibitor Cocktail (lyophilized)
• 10X Wash Buffer
• Protein A/G Sepharose Description:
Immunoprecipitation (IP) is widely used in research and development, by which a protein can be selectively purified from samples. BioVision’s immunoprecipitation kit provides optimized buffers for preparing cell/tissue extracts, antigen binding and washing steps. The protein A/G Sepharose beads provided in the kit has higher binding capacity with broader antibody isotype binding than traditional protein A or protein G resins. The kit can be used in variety of immunoprecipitation or Co-IP studies.

提供公司   萊恩生物科技有限公司

台中電話   04-25239639

聯絡業務   陳俊豪 0937-229803

電子信箱   biolion0819@gmail.com